✓ Accepted Answer
The way this question is framed suggests you might be hitting the same wall most people hit with crispr.
Let me work through the most likely causes from most to least common.
**Most likely culprit:** a misunderstanding of the core requirement. This accounts for roughly 56% of cases I have seen.
**Second possibility:** The approach you are using worked in a different context and you are trying to apply it where it does not fit. gene has specific conditions where it works well and conditions where it falls apart.
**Less common but worth checking:** a timing or sequence issue that only shows up under specific conditions.
To narrow it down: add logging or observation at each stage to see where things diverge. That will tell you which of these you are dealing with.
by frederickwalker
Honest take, because I wish someone had told me this earlier.
Everything you will read about crispr will make it sound more complicated than it is. Here is what 7 years of working with gene has actually taught me.
Everyone who's good at this now was terrible at it for longer than they'd admit.
What actually moved the needle for me: I stopped trying to understand everything before starting, and just committed to treating every mistake as data rather than failure. After that, things started moving much faster.
The one thing I would prioritise: do not compare your beginning to someone else's middle.
The learning curve is real but it is not as steep as it looks from the outside.
by fabianlouis8857